1RDD

CRYSTAL STRUCTURE OF ESCHERICHIA COLI RNASE HI IN COMPLEX WITH MG2+ AT 2.8 ANGSTROMS RESOLUTION: PROOF FOR A SINGLE MG2+ SITE

Experimental procedure

Spacegroup name	P 43 2 2
Unit cell lengths	63.200, 63.200, 80.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.800
R-factor	0.19
RMSD bond length	0.017
RMSD bond angle	0.044 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.095 *
Total number of observations	15785 *
Number of reflections	3953 *
Completeness [%]	90.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.6 (mg/ml)
2	1	drop		90-100 (mM)
3	1	drop	TAPS + CHES	50 (mM)
4	1	drop		25 (%sat)
5	1	reservoir		45 (%sat)