1RCX
NON-ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 218.300, 219.000, 113.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.23700 |
| Structure solution method | ISOMORPHOUS WITH PDB ENTRY 1RCO |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.328 |
| Total number of observations | 959052 * | |
| Number of reflections | 149428 | |
| <I/σ(I)> | 8.4 | 2.8 |
| Completeness [%] | 70.5 | 65 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | 4 * | 12% PEG 4000, 25 MM HEPES PH 7.8, 0.2 M NACL, 20 MM RUBP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | RuBP | 20 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 11-13 (%(w/v)) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | HEPES | 25 (mM) |
