1RCX

NON-ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	218.300, 219.000, 113.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.400
R-factor	0.224
Rwork	0.224
R-free	0.23700
Structure solution method	ISOMORPHOUS WITH PDB ENTRY 1RCO
RMSD bond length	0.011
RMSD bond angle	23.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.328
Total number of observations	959052 *
Number of reflections	149428
<I/σ(I)>	8.4	2.8
Completeness [%]	70.5	65
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8	4 *	12% PEG 4000, 25 MM HEPES PH 7.8, 0.2 M NACL, 20 MM RUBP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	RuBP	20 (mM)
3	1	reservoir	PEG4000	11-13 (%(w/v))
4	1	reservoir		0.2 (M)
5	1	reservoir	HEPES	25 (mM)