1RCT
Crystal structure of Human purine nucleoside phosphorylase complexed with INOSINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 104 |
| Detector technology | CCD |
| Collection date | 2003-07-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.431 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 140.990, 140.990, 161.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pwy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 25.250 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.790 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.072 | 0.268 * |
| Number of reflections | 15363 | |
| Completeness [%] | 93.3 | 95.7 |
| Redundancy | 5.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.1 * | 25 * | pH 5.30 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 10 (mM) | pH7.1 |
| 3 | 1 | reservoir | ammonium sulfate | 19 (%(v/v)sat) | |
| 4 | 1 | reservoir | citrate | 0.05 (M) | pH5.3 |
