1RCM
CRYSTAL STRUCTURE OF A UBIQUITIN-DEPENDENT DEGRADATION SUBSTRATE: A THREE-DISULFIDE FORM OF LYSOZYME
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.700, 81.300, 38.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.800 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 * |
| High resolution limit [Å] | 1.900 * |
| Number of reflections | 18295 * |
| Completeness [%] | 93.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 3.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | reservoir | 7 (%) | ||
| 3 | 1 | reservoir | sodium acetate | 50 (mM) |
