1RCF

STRUCTURE OF THE TRIGONAL FORM OF RECOMBINANT OXIDIZED FLAVODOXIN FROM ANABAENA 7120 AT 1.40 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	55.360, 55.360, 102.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 1.400
R-factor	0.139
Rwork	0.139
RMSD bond length	0.015
RMSD bond angle	0.015 *
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.400 *	1.400 *
Rmerge	0.038 *
Number of reflections	36069	6774 *
Completeness [%]	99.1	94.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		277 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	80 (mg/ml)
2	1	drop	Tris-HCl	200 (mM)
3	1	drop	ammonium sulfate	80 (%sat)
4	1	reservoir	ammonium sulfate	3.3 (M)