1RCB

CRYSTAL STRUCTURE OF HUMAN RECOMBINANT INTERLEUKIN-4 AT 2.25 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	91.800, 91.800, 46.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.250
R-factor	0.218
RMSD bond length	0.020
RMSD bond angle	0.056
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.230 *
Rmerge	0.060 *
Number of reflections	8951 *
Completeness [%]	90.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	K-phosphate	50 (mM)
3	1	reservoir	ammonium sulfate	60-63 (%)
4	1	reservoir	K-phosphate	50 (mM)