1RC7
Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-05 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.286, 118.142, 106.665 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.470 - 2.150 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1I4S |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.470 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.058 | 0.262 |
| Number of reflections | 18715 | |
| <I/σ(I)> | 22 | 1.8 |
| Completeness [%] | 91.6 | 71.2 |
| Redundancy | 5.05 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | PEG4000, Tris-HCl, Sodium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | Tris-HCl | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 2 | NaCl | ||
| 5 | 1 | 2 | PEG4000 |
