1RBW

RIBONUCLEASE A (E.C.3.1.27.5) WITH GUANIDINIUM

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1994-07-15
Detector	RIGAKU AFC-5R
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	30.327, 38.412, 53.143
Unit cell angles	90.00, 105.83, 90.00

Refinement procedure

Resolution	10.000 - 1.690
R-factor	0.191 *
Rwork	0.190
R-free	0.20600
RMSD bond length	0.010
RMSD bond angle	2.300 *
Data reduction software	TEXSAN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.690
Rmerge	0.090
Total number of observations	17117 *
Number of reflections	10870
Completeness [%]	99.0
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	L-histidine	0.5 (M)
2	1	drop	calcium acetate	0.5 (M)
3	1	reservoir	Tris	0.1 (M)
4	1	reservoir	ethanol	6.5 (%(v/v))