1RBL

STRUCTURE DETERMINATION AND REFINEMENT OF RIBULOSE 1,5 BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM SYNECHOCOCCUS PCC6301

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	223.900, 111.900, 199.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 * - 2.200
R-factor	0.2
Rwork	0.200
RMSD bond length	0.008
RMSD bond angle	26.120 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.105 *
Total number of observations	852051 *
Number of reflections	218276 *
Completeness [%]	86.2 *	51.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *	Newman, J., (1990) J. Biol. Chem., 265, 15154. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme solution	30-40 (mg/ml)
2	1	drop	PEG4000	12-16 (%)
3	1	drop	phosphate	25 (mM)
4	1	drop		1 (mM)
5	1	reservoir	PEG4000	12-16 (%)
6	1	reservoir	phosphate	25 (mM)
7	1	reservoir		1 (mM)