1RB8
The phiX174 DNA binding protein J in two different capsid environments.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 295.143, 295.143, 678.235 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 92.910 - 3.500 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Bacteriophage alpha3 wild-type structure |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 3.660 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.097 | 0.157 |
| Number of reflections | 269234 | |
| Completeness [%] | 96.9 | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 4-7% PEG 8000, 100 mM sodium citrate pH 5.0, 40% glycerol, 0.02% sodium azide, 0.1% beta-mercapto-ethanol, 0.9M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 10 | 1 | 2 | glycerol | ||
| 11 | 1 | 2 | NaCl | ||
| 2 | 1 | 1 | sodium citrate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | sodium azide | ||
| 5 | 1 | 1 | beta-mercapto-ethanol | ||
| 6 | 1 | 1 | NaCl | ||
| 7 | 1 | 1 | water | ||
| 8 | 1 | 2 | PEG 8000 | ||
| 9 | 1 | 2 | sodium citrate |
