1RAT
EFFECTS OF TEMPERATURE ON PROTEIN STRUCTURE AND DYNAMICS: X-RAY CRYSTALLOGRAPHIC STUDIES OF THE PROTEIN RIBONUCLEASE-A AT NINE DIFFERENT TEMPERATURES FROM 98 TO 320 K
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.480, 38.080, 52.970 |
| Unit cell angles | 90.00, 106.09, 90.00 |
Refinement procedure
| Resolution | ? - 1.500 |
| R-factor | 0.171 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.034 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.500 * |
| Rmerge | NaN * |
| Number of reflections | 11214 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.5 * | 20 * | Tilton Jr., R.F., (1992) Biochemistry, 31, 2469. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 12 (mg/ml) | |
| 2 | 1 | 1 | 0.2 (M) | ||
| 3 | 1 | 1 | methanol | 50 (%(v/v)) |
