1RAS

CRYSTAL STRUCTURE OF A FLUORESCENT DERIVATIVE OF RNASE A

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	65.100, 65.100, 65.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.203
RMSD bond length	0.011
RMSD bond angle	0.036 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.074 *
Total number of observations	53253 *
Number of reflections	19015 *
Completeness [%]	89.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.1 *	20 *	CSCL WAS WASHED OUT IN 80% AMMONIUM SULFATE BEFORE DATA COLLECTION.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		3 (M)
2	1	drop	ammonium sulfate	80 (%)
3	1	reservoir	acetate	50 (mM)