1R8J
Crystal Structure of Circadian Clock Protein KaiA from Synechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.111, 125.818, 56.818 |
| Unit cell angles | 90.00, 114.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.030 |
| R-factor | 0.21306 |
| Rwork | 0.210 |
| R-free | 0.27144 |
| Structure solution method | combination of SAD phasing, real space molecular replacement, ARP/wARP phase improvement, and non-crystallographic symmetry averaging |
| Starting model (for MR) | 1m2e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.362 |
| Number of reflections | 38494 | |
| <I/σ(I)> | 30.6 | 2.7 |
| Completeness [%] | 95.2 | 54.3 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 298 | Ammonium Sulfate, Sodium Cacodylate, PEG 8000, Glycerol, pH 6.5, EVAPORATION, temperature 298.0K |
