1R8D
Crystal Structure of MtaN Bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 4 |
| Unit cell lengths | 148.190, 148.190, 73.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 2.700 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.26900 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.800 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 21769 | |
| <I/σ(I)> | 22.2 | 1.8 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 1450, lithium sulfate, PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 1450 | ||
| 2 | 1 | 1 | lithium sulfate | ||
| 3 | 1 | 1 | PIPES | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 1450 | ||
| 6 | 1 | 2 | lithium sulfate | ||
| 7 | 1 | 2 | H2O |
