1R88
The crystal structure of Mycobacterium tuberculosis MPT51 (FbpC1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.900, 108.900, 109.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 * |
| R-factor | 0.18954 |
| Rwork | 0.189 |
| R-free | 0.20500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.077 * | 0.489 * |
| Total number of observations | 493963 * | |
| Number of reflections | 78268 | |
| <I/σ(I)> | 22 | 3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 8-10 (%(w/v)) | |
| 2 | 1 | reservoir | MPD | 8-10 (%(v/v)) | |
| 3 | 1 | reservoir | isopropanol | 2.5 (%(v/v)) | |
| 4 | 1 | reservoir | sodium acetate | 200 (mM) | pH4.6 |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.0 |
| 6 | 1 | drop | protein | 70 (mg/ml) |
