1R86
Crystal structure of the extracellular xylanase from Geobacillus stearothermophilus T-6 (XT6, monoclinic form): The E159A/E265A mutant at 1.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-25 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.244, 61.251, 89.463 |
| Unit cell angles | 90.00, 119.28, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.21500 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | PDB code 1R85 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.481 |
| Number of reflections | 48254 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 90.9 | 90.5 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% PEG 4000, MES buffer pH 6.5, 10mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
