1R7L
2.0 A Crystal Structure of a Phage Protein from Bacillus cereus ATCC 14579
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.9798, 0.9465 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.002, 66.338, 53.966 |
| Unit cell angles | 90.00, 95.29, 90.00 |
Refinement procedure
| Resolution | 24.900 - 2.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.098 | 0.518 |
| Number of reflections | 17557 | |
| <I/σ(I)> | 22.3 | 2.25 |
| Completeness [%] | 99.1 | 95.6 |
| Redundancy | 8.12 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 20% PEG 8000, 0.1M MES, 0.2M Ca(OAc)2, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
