1R75
Leishmania major hypothetical protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2003-08-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.629, 57.629, 84.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.770 - 1.860 |
| Rwork | 0.201 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.096 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (refmac_5.1.24 24/04/2001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 12293 | |
| <I/σ(I)> | 16.5 | 2.5 |
| Completeness [%] | 97.3 | 80.8 |
| Redundancy | 6.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 298 | pH 7.6, sitting drop vapor diffusion, temperature 298K, pH 7.60 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Lmaj002144AAA | ||
| 2 | 1 | 1 | Na/K Phosphate | ||
| 3 | 1 | 1 | Hepes Buffer |
