1R71
Crystal Structure of the DNA binding domain of KorB in complex with the operator DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92022, 0.92039, 0.89844 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 110.440, 110.440, 160.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.19759 |
| Rwork | 0.195 |
| R-free | 0.25028 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.012 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 57669 | |
| <I/σ(I)> | 14.91 | |
| Completeness [%] | 92.7 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293.15 | 25% MPD, 0.4M ammonium dihydrogen phosphate, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | ammonium dihydrogen phosphate | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | H2O |
