1R6O
ATP-dependent Clp protease ATP-binding subunit clpA/ATP-dependent Clp protease adaptor protein clpS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2823 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 87.436, 87.436, 212.955 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.19022 |
| Rwork | 0.190 |
| R-free | 0.20546 |
| Structure solution method | MIR |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.025 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.049 | 0.680 |
| Number of reflections | 45504 | |
| <I/σ(I)> | 27.7 | 1.75 |
| Completeness [%] | 1.0 | 0.94 |
| Redundancy | 4.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 294 | Drop: 0.02M Tris HCl (pH 8.5), 2mM ZnCl2, 10% (w/v) glycerol; Precipitant: 0.1M bis-tris (pH 6.5), 32% (w/v) glycerol, 0.01-0.015M yttrium chloride, HANGING DROP, temperature 294K |
