1R6M

Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa In Complex With Phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2003-05-28
Detector	MACSCIENCE
Wavelength(s)	1.1272
Spacegroup name	H 3 2
Unit cell lengths	110.202, 110.202, 116.284
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 2.000
R-factor	0.226
Rwork	0.226
R-free	0.27900 *
Structure solution method	difference Fourier
RMSD bond length	0.006
RMSD bond angle	23.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.039 *	0.332 *
Total number of observations	120641 *
Number of reflections	18518
Completeness [%]	94.6	89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	18 *	Ammonium phosphate, citrate, sodium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)	pH8.0
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	reservoir	ammonium sulfate	1.2 (M)
6	1	reservoir		0.1 (M)
7	1	reservoir	CHES	0.2 (M)	pH9.5