1R6M
Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa In Complex With Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-28 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.1272 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.202, 110.202, 116.284 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27900 * |
| Structure solution method | difference Fourier |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.039 * | 0.332 * |
| Total number of observations | 120641 * | |
| Number of reflections | 18518 | |
| Completeness [%] | 94.6 | 89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 18 * | Ammonium phosphate, citrate, sodium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
| 6 | 1 | reservoir | 0.1 (M) | ||
| 7 | 1 | reservoir | CHES | 0.2 (M) | pH9.5 |
