1R62
Crystal structure of the C-terminal Domain of the Two-Component System Transmitter Protein NRII (NtrB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.440, 32.940, 46.010 |
| Unit cell angles | 90.00, 108.19, 90.00 |
Refinement procedure
| Resolution | 27.610 - 1.600 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.039 | |
| Number of reflections | 18060 | |
| <I/σ(I)> | 35 | 4.2 |
| Completeness [%] | 98.8 | 95.3 |
| Redundancy | 4.05 | 3.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 298 | 5% PEG 20,000 and 0.1M MES, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
