1R5Z
Crystal Structure of Subunit C of V-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 61 |
| Unit cell lengths | 118.429, 118.429, 152.086 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.19359 |
| Rwork | 0.191 |
| R-free | 0.24900 * |
| Structure solution method | SIR |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.936 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.068 | 0.328 * |
| Total number of observations | 410472 * | |
| Number of reflections | 86730 | 8633 * |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.3 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 4 * | MPD, ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 2 | 1 | reservoir | MPD | 22-26 (%) | |
| 3 | 1 | reservoir | ammonium acetate | 0.2 (M) |
