1R5Z

Crystal Structure of Subunit C of V-ATPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-07
Detector	MARRESEARCH
Wavelength(s)	0.95
Spacegroup name	P 61
Unit cell lengths	118.429, 118.429, 152.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.950
R-factor	0.19359
Rwork	0.191
R-free	0.24900 *
Structure solution method	SIR
RMSD bond length	0.025
RMSD bond angle	1.936
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.068	0.328 *
Total number of observations	410472 *
Number of reflections	86730	8633 *
<I/σ(I)>	13.8
Completeness [%]	99.3	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6	4 *	MPD, ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.1 (M)	pH5.6
2	1	reservoir	MPD	22-26 (%)
3	1	reservoir	ammonium acetate	0.2 (M)