1R5X

JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9790, 0.9792, 0.9184
Spacegroup name	P 65
Unit cell lengths	76.161, 76.161, 92.124
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.190 - 2.300
R-factor	0.263
Rwork	0.263
R-free	0.30400 *
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.094 *	0.379 *
Total number of observations	278220 *
Number of reflections	13506
<I/σ(I)>	4.7	1.8
Completeness [%]	99.9
Redundancy	20.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	ammonium di-hydrogen phosphate, sodium citrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5	293	ammonium di-hydrogen phosphate, sodium citrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5	293	ammonium di-hydrogen phosphate, sodium citrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		100 (mM)
2	1	reservoir	sodium cirtate	200 (mM)	pH5.
3	1	drop	protein	30 (mg/ml)