1R5M

Crystal Structure Of The C-Terminal WD40 Domain Of Sif2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-12-16
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 65
Unit cell lengths	107.100, 107.100, 82.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.780 - 1.550
R-factor	0.19
Rwork	0.190
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Used model from a in lab MAD solved structure of C-Sif2
RMSD bond length	0.004
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.550	1.550
Number of reflections	75148
<I/σ(I)>	26.5	3.17
Completeness [%]	99.8	99.8
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100mM Mes, 0.2M ammonium sulfate, 30% PEG ME 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K