1R5I
Crystal structure of the MAM-MHC complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 138.286, 178.814, 179.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 * - 2.600 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.24600 |
| Structure solution method | SAD and molecular replacement |
| Starting model (for MR) | 1dlh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.900 * |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.489 |
| Number of reflections | 65311 | |
| <I/σ(I)> | 15 | 2.1 |
| Completeness [%] | 95.9 * | 80.4 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | potassium sodium hosphate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.4 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | reservoir | potassium sodium phosphate | 1.7 (M) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
