1R5A
Glutathione S-transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 122.129, 122.129, 74.699 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| Rwork | 0.233 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v2a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 20.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 0.396 |
| Total number of observations | 37582 * | |
| Number of reflections | 9605 | 963 * |
| <I/σ(I)> | 12.9 | 2.6 |
| Completeness [%] | 95.6 | 97.9 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.75M lithium sulfate, 0.1M potassium phosphate, 1mM Copper (II) chloride, 10mM glutathione-sulfonic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.6-2 (M) | ||
| 2 | 1 | reservoir | 1 (mM) | ||
| 3 | 1 | reservoir | sodium phosphate | 100 (mM) | pH6.5-7.5 |
| 4 | 1 | drop | protein | 13.1 (mg/ml) | |
| 5 | 1 | drop | glutathione sulphonic acid | 10 (mM) |
