1R4X

Crystal Structure Analys of the Gamma-COPI Appendage domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Wavelength(s)	1.9100
Spacegroup name	P 21 21 21
Unit cell lengths	48.800, 74.100, 99.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.17454
Rwork	0.173
R-free	0.20800 *
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.480 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.082	0.281
Number of reflections	29073
<I/σ(I)>		6.7
Completeness [%]	96.9	96.3
Redundancy	6.7	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	299	PEG 8000, Magnesium Acetate, Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 299K
1	VAPOR DIFFUSION, SITTING DROP	6.5	299	PEG 8000, Magnesium Acetate, Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 299K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	dithiothreitol	5 (mM)
2	1	drop	protein	10-15 (mg/ml)
3	1	reservoir	magnesium acetate	0.2 (M)
4	1	reservoir	cacodylate	0.1 (M)	pH6.5
5	1	reservoir	PEG8000	20 (%)