1R4O
Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 263 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.500, 97.500, 120.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.670 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 14846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | MPD, sodium cacodylate, sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | sodium chloride | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | sodium cacodylate | ||
| 7 | 1 | 2 | H2O |
