1R3Y
Uroporphyrinogen Decarboxylase in complex with coproporphyrinogen-III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 103.140, 103.140, 74.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.710 - 1.755 |
| R-factor | 0.15905 |
| Rwork | 0.158 |
| R-free | 0.18574 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1uro |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.627 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.710 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.091 | 0.423 |
| Number of reflections | 45954 | |
| <I/σ(I)> | 11 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 294 | PBG, PBG-D and U3S were added to the protein solution in an anaerobic chamber. URO-D was active under these conditions, converting uro'gen-III to cop'gen-III. 1.5 M citrate, pH 6.5, LIQUID DIFFUSION, temperature 294K |
