1R3O
Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 46.109, 46.109, 263.501 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 1.900 |
| R-factor | 0.24082 |
| Rwork | 0.239 |
| R-free | 0.28600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.766 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 1.900 | |
| Rmerge | 0.064 * | 0.470 * |
| Number of reflections | 8949 * | 511 * |
| Completeness [%] | 98.7 * | 98.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | pH 7.0, temperature 100K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MPD | 10 (%(v/v)) | |
| 2 | 1 | drop | 80 (mM) | ||
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | spermine tetrahydrochloride | 12 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 40 (mM) | pH6.0 |
| 6 | 1 | drop | RNA | 0.25 (mM) | |
| 7 | 1 | reservoir | MPD | 30 (%(v/v)) |
