1R3O

Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	46.109, 46.109, 263.501
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 1.900
R-factor	0.24082
Rwork	0.239
R-free	0.28600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.766
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.900
Rmerge	0.064 *	0.470 *
Number of reflections	8949 *	511 *
Completeness [%]	98.7 *	98.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *		pH 7.0, temperature 100K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MPD	10 (%(v/v))
2	1	drop		80 (mM)
3	1	drop		20 (mM)
4	1	drop	spermine tetrahydrochloride	12 (mM)
5	1	drop	sodium cacodylate	40 (mM)	pH6.0
6	1	drop	RNA	0.25 (mM)
7	1	reservoir	MPD	30 (%(v/v))