1R3I
potassium channel KcsA-Fab complex in Rb+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-17 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 1.1 |
Spacegroup name | I 4 |
Unit cell lengths | 155.840, 155.840, 75.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.218 |
Rwork | 0.217 |
R-free | 0.23800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
RMSD bond length | 0.016 |
RMSD bond angle | 24.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.074 | 0.326 |
Number of reflections | 33943 | |
<I/σ(I)> | 15.2 | 2.6 |
Completeness [%] | 99.1 | 93.7 |
Redundancy | 3.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 50 (mM) | pH7.5 |
2 | 1 | drop | DM | 5 (mM) | |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | reservoir | PEG400 | 18-25 (%(w/v)) | |
5 | 1 | reservoir | magnesium acetate | 50 (mM) | |
6 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.4 |