1R3C
THE STRUCTURE OF P38ALPHA C162S MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.162, 84.799, 124.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1wfc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.200 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.410 |
| Number of reflections | 33222 | 5440 * |
| <I/σ(I)> | 9.6 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | SODIUM CITRATE, AMMONIUM SULFATE, HEPES, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.4 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | glycerol | 5 (%) | |
| 5 | 1 | drop | TCEP | 2 (mM) | |
| 6 | 1 | reservoir | sodium citrate | 0.62-0.69 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 130 (mM) | |
| 8 | 1 | reservoir | HEPES | 40 (mM) | pH7.5 |
