1R2Y
MutM (Fpg) bound to 8-oxoguanine (oxoG) containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.290, 93.634, 102.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.340 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1l1t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.500 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.090 | 0.395 |
| Number of reflections | 18601 | |
| <I/σ(I)> | 22.9 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 298 | Fromme, J.C., (2002) Nat.Struct.Biol., 9, 544. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | MAGNESIUM ACETATE | ||
| 3 | 1 | 1 | SODIUM CACODYLATE | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 8000 | ||
| 6 | 1 | 2 | MAGNESIUM ACETATE | ||
| 7 | 1 | 2 | SODIUM CACODYLATE | ||
| 8 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH7.4 |
| 2 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | protein | 8 (mg/ml) | |
| 5 | 1 | reservoir | PEG8000 | 13-18 (%(w/v)) | |
| 6 | 1 | reservoir | sodium cacodylate | 100 (mg/ml) | pH7.5 |
| 7 | 1 | reservoir | magnesium acetate | 50 (mM) | |
| 8 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%) |
