1R28

Crystal Structure of the B-Cell Lymphoma 6 (BCL6) BTB domain to 2.2 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-10-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	140.750, 32.660, 48.630
Unit cell angles	90.00, 94.73, 90.00

Refinement procedure

Resolution	30.000 * - 2.200
R-factor	0.2
Rwork	0.200
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.360	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.092 *	0.281 *
Number of reflections	10909
<I/σ(I)>	12.8	3.2
Completeness [%]	95.0	85.3
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 8000, sodium acetate, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	30 (%)
2	1	reservoir	sodium acetate	0.18 (M)
3	1	reservoir	HEPES	0.1 (M)	pH7.0