1R24

FAB FROM MURINE IGG3 KAPPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	270
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	139.240, 82.070, 73.580
Unit cell angles	90.00, 94.13, 90.00

Refinement procedure

Resolution	6.000 - 3.100
R-factor	0.245
Rwork	0.245
R-free	0.33400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FAB FROM YST9.1
RMSD bond length	0.022
RMSD bond angle	3.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MERLOT
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	3.100
Rmerge	0.075 *
Total number of observations	58762 *
Number of reflections	26595
Completeness [%]	92.0
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	23 (%)
2	1	reservoir	zinc acetate	0.2 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)