1R1V

Crystal structure of the metal-sensing transcriptional repressor CzrA from Staphylococcus aureus in the Zn2-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	54.444, 75.370, 50.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 2.300
R-factor	0.205
Rwork	0.195
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r1t
RMSD bond length	0.005
RMSD bond angle	1.017
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	9503
<I/σ(I)>	15.4
Completeness [%]	97.4	91.4
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	PEG 8000, NaCl, Ches, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K