1R1G
Crystal Structure of the Scorpion Toxin BmBKTtx1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5478 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 21.397, 39.695, 29.368 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.720 |
| Rwork | 0.166 |
| R-free | 0.22400 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.000 * |
| Data reduction software | XPREP |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.036 | 0.089 |
| Total number of observations | 9834 * | |
| Number of reflections | 5064 * | |
| <I/σ(I)> | 65.5 | 19 |
| Completeness [%] | 95.4 | 91.6 |
| Redundancy | 12.6 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sparse-matrix * | 293 | Sodium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | protein | 100 (mg/ml) |
