1R13
Carbohydrate recognition and neck domains of surfactant protein A (SP-A)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 63 |
| Unit cell lengths | 97.680, 97.680, 44.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.212 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.299 |
| Total number of observations | 189251 * | |
| Number of reflections | 14373 | |
| <I/σ(I)> | 25.4 | 6.6 |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 17 * | LiSO4, CaCl2, glycerol, trimannose, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 5 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | protein | 3 (mg/ml) | |
| 4 | 1 | reservoir | 1.6 (M) | ||
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | glycerol | 7 (%(w/v)) | |
| 7 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
