1R0V
Structure Determination of the Dimeric Endonuclease in a Pseudo-face-centerd P21212 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 128.119, 144.149, 52.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.350 - 2.000 |
| R-factor | 0.1771 |
| Rwork | 0.174 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.043 |
| RMSD bond angle | 3.288 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 60298 | |
| Completeness [%] | 90.8 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 100 mM Na-cacodylic acid, pH 6.5 - 6.8, 20 mM (NH4)2SO4, 300-400 mM NaCH2COOH, pH 6.6 |
