1R0N
Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2834, 1.2837, 1.2155 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.620, 60.260, 115.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.600 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28200 * |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.102 * | 0.224 |
| Number of reflections | 11884 * | |
| <I/σ(I)> | 27.5 | 6.7 |
| Completeness [%] | 94.2 | 75.4 * |
| Redundancy | 9.8 * | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 8 * | Zhao, Q., (2000) J.Mol.Biol., 296, 509. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 10 | 1 | 2 | DTT | ||
| 11 | 1 | 2 | Magnesium Chloride | ||
| 12 | 1 | 2 | MES | ||
| 13 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Lithium Sulfate | ||
| 3 | 1 | 1 | Magnesium Sulfate | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | Magnesium Chloride | ||
| 6 | 1 | 1 | MES | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | PEG 3350 | ||
| 9 | 1 | 2 | Magnesium Sulfate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 25 (mM) | |
| 2 | 1 | drop | protein | 0.9 (mM) | |
| 3 | 1 | drop | DNA | 0.45 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 18-23 (%) | |
| 5 | 1 | reservoir | Tris | 25 (mM) | |
| 6 | 1 | reservoir | 5 (mM) | ||
| 7 | 1 | reservoir | 400 (mM) |
