1R03
crystal structure of a human mitochondrial ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.966 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.410, 181.410, 181.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.900 - 1.700 |
| R-factor | 0.18 |
| Rwork | 0.170 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fha |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.570 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.900 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 25592 | |
| <I/σ(I)> | 4.91 | 2 |
| Completeness [%] | 87.8 | 65.3 |
| Redundancy | 5.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Tris HCL, magnesium chloride, sodium chloride, bicine, sodium azide, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
