1QZV
Crystal structure of plant photosystem I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 182.283, 190.376, 220.253 |
| Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.440 |
| R-factor | 0.41 |
| Rwork | 0.410 |
| R-free | 0.42000 |
| Structure solution method | MIR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.520 |
| High resolution limit [Å] | 4.440 | 4.440 |
| Rmerge | 0.099 | 0.860 |
| Number of reflections | 92318 | |
| <I/σ(I)> | 18.8 | 2.05 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 277 | Ben-Shem, A., (2003) Acta Cryst., D59, 1824. * |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 277 | Ben-Shem, A., (2003) Acta Cryst., D59, 1824. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | MES-bis-tris | 22.5 (mM) | pH6.6 |
| 3 | 1 | reservoir | PEG400 | 0.5 (%(v/v)) | |
| 4 | 1 | reservoir | ammonium citrate | 8.1 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 5.6-6.4 (%(w/v)) |
