1QZN
Crystal Structure Analysis of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.088, 55.088, 112.553 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.670 - 1.900 |
| R-factor | 0.16133 |
| Rwork | 0.159 |
| R-free | 0.19868 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.021 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.680 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 0.662 |
| Number of reflections | 16118 | |
| <I/σ(I)> | 20.8 | 2.016 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | ammonium sulfate, sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
