1QZ6

Structure of rabbit actin in complex with jaspisamide A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	40.370, 75.958, 67.494
Unit cell angles	90.00, 99.07, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.16689
Rwork	0.165
R-free	0.20100 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.680 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.044	0.303
Total number of observations	237001 *
Number of reflections	52492
<I/σ(I)>	37	5.2
Completeness [%]	99.1	96.8 *
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7	293	Crystals were grown by small-scale batch by mixing equal volumes of the complex (10 mg/ml protein) and 100 mM bis-tris propane, 15% (w/v) dimethyl polyethylene glycol 5000, 25 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 7.0, Batch, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10-12 (mg/ml)
2	1	1	bis-tris propane	100 (mM)	pH7.0
3	1	1	dimethyl polyethylene glycol5000	15 (%(w/v))
4	1	1		25 (mM)
5	1	1		1 (mM)
6	1	1	TCEP	1 (mM)