1QZ5
Structure of rabbit actin in complex with kabiramide C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.919, 70.617, 75.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.17001 |
| Rwork | 0.169 |
| R-free | 0.18800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.670 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.044 | 0.123 |
| Total number of observations | 542898 * | |
| Number of reflections | 65708 | |
| <I/σ(I)> | 47.1 | 13.9 |
| Completeness [%] | 98.5 | 95.7 * |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.5 | 293 | Crystals of the actin-kabiramide C complex were grown by small-scale batch by mixing equal volumes of the complex and 100 mM MES, 18% (w/v) polyethylene glycol 1500, 12% (w/v) 1,6-hexanediol, 100 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 5.5, Batch, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10-12 (mg/ml) | |
| 2 | 1 | 1 | MES | 100 (mM) | pH5.5 |
| 3 | 1 | 1 | PEG1500 | 18 (%(w/v)) | |
| 4 | 1 | 1 | 1,6-hexanediol | 12 (%(w/v)) | |
| 5 | 1 | 1 | 100 (mM) | ||
| 6 | 1 | 1 | 1 (mM) | ||
| 7 | 1 | 1 | TCEP | 1 (mM) |
