1QYK
GCATGCT + Barium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8416 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 22.015, 59.536, 47.667 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.400 |
| R-factor | 0.2011 |
| Rwork | 0.201 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB entry UDG028 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.073 | 0.256 |
| Number of reflections | 6514 | |
| <I/σ(I)> | 4.94 | |
| Completeness [%] | 98.5 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 290 | Sodium Cacodylate, Barium Chloride, Potassium Chloride, MPD, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Cacodylate | ||
| 2 | 1 | 1 | Barium Chloride | ||
| 3 | 1 | 1 | Potassium Chloride | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | Sodium Cacodylate | ||
| 7 | 1 | 2 | Barium Chloride | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
