1QY7

The structure of the PII protein from the cyanobacteria Synechococcus sp. PCC 7942

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2002-04-02
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	I 41 2 2
Unit cell lengths	109.700, 109.700, 109.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.000
Rwork	0.199
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pii
RMSD bond length	0.005
RMSD bond angle	23.640 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.059	0.241
Total number of observations	68888 *
Number of reflections	19247
<I/σ(I)>	8.5	3.1
Completeness [%]	84.0 *	80.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Lithium sulfate, Nickel chloride, TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	lithium sulfate	1.0 (M)
3	1	reservoir	Tris	0.1 (M)	pH8.5
4	1	reservoir	nickel chloride hexahydrate	10 (mM)