1QXT
Crystal structure of precyclized intermediate for the green fluorescent protein R96A variant (A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.78 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.928, 61.571, 69.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.012 |
| RMSD bond angle | 28.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 * | 0.372 * |
| Total number of observations | 54856 * | |
| Number of reflections | 15550 | |
| <I/σ(I)> | 18.2 | 3.3 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 3.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | used microseeding., Barondeau, D.P., (2002) J.Am.Chem.Soc., 124, 3522. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 50 (mM) | pH8.0 |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 19 (%) |
