1QX8
Crystal structure of a five-residue deletion mutant of the Rop protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-05-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 54.476, 42.565, 51.722 |
| Unit cell angles | 90.00, 104.68, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.020 |
| R-factor | 0.19057 |
| Rwork | 0.189 |
| R-free | 0.21218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rpo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.394 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.066 | 0.312 |
| Number of reflections | 7271 | |
| <I/σ(I)> | 7.8 | 2.2 |
| Completeness [%] | 95.3 | 76 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | NaCl, methanol, Bis-Tris, DTT, EDTA, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
